Apoptosis Imaging: Beyond Annexin V

Apoptosis imaging: beyond annexin V.

Induction of apoptosis is the primary mechanism through which most chemotherapies cause tumor cell death. Early assessment of tumor response is required to manage patients in terms of quality of life versus intensive chemotherapy. Although imaging with radiolabeled annexin V has been intensively investigated, it is still not sufficiently mature for clinical application. This article will summar...

Iodination of annexin V for imaging apoptosis.

UNLABELLED Our goal in this investigation was to develop a method for iodinating annexin V that would be suitable for the in vivo detection of apoptosis. METHODS Annexin V was iodinated with (125)I using 2 different techniques: direct iodination with IODO-BEADS, resulting in the iodination of tyrosine residues; and use of the Bolton-Hunter reagent, which binds to lysine. The active fraction o...

Annexin V imaging of acute doxorubicin cardiotoxicity (apoptosis) in rats.

UNLABELLED Anthracyclines are widely used in chemotherapy regimens for several malignancies, with cardiotoxicity being the major limiting factor in high-dose schedules. Recently, it was reported that doxorubicin induces apoptosis in cardiac muscle cells in vivo and, as such, is expected to be involved in the genesis of doxorubicin-induced cardiomyopathy. The aim of this study was to validate an...

Annexin V Delays Apoptosis While Exerting

SPIO-Annexin V, a potential probe for MRI detection of radiation induced apoptosis

Background: Finding a suitable method for rapid, accurate and reliable estimation of absorbed dose has high priority in management of the radiation exposed persons. Shortly after radiation exposure, apoptosis is a major detriment in proliferative tissues such as the hematopoietic system. Therefore, quantification of apoptosis in these tissues could be useful for rapid estimation of radiation ex...